Metabolic Blend: structure and analysis

What is Metabolic Blend?

A three-compound blend supplied in a single vial: NAD+ at 100 mg, MOTS-c at 10 mg, and 5-Amino-1MQ at 10 mg. The notation 100/10/10mg gives each component's mass in that order.

Two of the three components are not peptides. NAD+ — nicotinamide adenine dinucleotide — is a coenzyme built from two nucleotides. 5-Amino-1MQ is a small-molecule quinolinium. Only MOTS-c, a 16-residue mitochondrially encoded peptide, is a peptide at all.

This makes it the only product in the catalogue that is not predominantly peptide material, and the distinction is not cosmetic: peptide-specific analytical methods do not apply uniformly across the vial.

How is Metabolic Blend produced?

Supplied as a blend: the named components are combined and co-lyophilized into a single vial, with the mass of each component stated. The components are produced separately and characterised before combination.

This changes the analytical question. For a single compound, purity asks what share of the detected material was the target. For a blend there are several targets, and what matters is whether each named component is present at its stated mass — per-component identification and quantitation rather than one purity percentage.

What is in Metabolic Blend?

Metabolic Blend is supplied as one co-lyophilized vial holding 3 separately characterised components, in the ratio 100/10/10mg. A blend has no single molecular formula, because a formula describes one molecule and this vial holds several, so the table gives each component's own published figures.

For a neutral molecule the ion a mass spectrometer is compared against is the protonated species, [M+H]⁺. Two of these components are already charged — a copper complex and a quaternary ammonium — and for those the ion to compare against is the species itself; the table says which is which.

Two masses describe each of these molecules and they are different numbers: the monoisotopic mass, which assumes every carbon is carbon-12, and the tallest peak in the isotope envelope, which for a long peptide sits one or two carbon-13 substitutions above it. The table gives the monoisotopic figure; each component's own page gives both.

What a certificate can establish for a vial like this is a separate identity and a separate quantitation for each named component, each against a standard appropriate to that molecule. One purity percentage does not describe several targets, and the certificate has to be read per analyte rather than as one number.

How does Metabolic Blend compare with related compounds?

Related compounds are not interchangeable. Published figures differ between them, and in this catalogue three compounds share a GHRH backbone while several share the same structural class — which is exactly why identity is confirmed analytically rather than inferred from the label.

How is the identity of Metabolic Blend confirmed?

Three components requiring three different analytical approaches. MOTS-c is confirmed as a peptide would be, by mass spectrometry against the expected 16-residue mass. NAD+ and 5-Amino-1MQ are confirmed as small molecules, against their own reference standards.

A certificate that applies peptide methods across this vial has characterised one third of its contents.

Which impurities can appear in Metabolic Blend?

The methods diverge more here than for any other product. Reversed-phase HPLC with far-ultraviolet detection is well suited to a peptide; NAD+ is a highly polar nucleotide with strong absorbance near 260 nm, and a quinolinium is a small aromatic cation. Their chromatographic and detection requirements genuinely differ.

Purity in the peptide sense is not a meaningful single figure for this vial. Per-component identification and quantitation against appropriate reference standards is the only coherent way to characterise it.

Stability is component-dependent too. NAD+ is notably less stable in solution than a lyophilized peptide, so the vial's storage conditions are set by its least stable component — and in this case that is unlikely to be the peptide.

What does a certificate of analysis for Metabolic Blend report?

A certificate for a batch of this material names the batch, names the issuing laboratory, and states for each parameter both the method used and the specification the result was measured against. Identity and purity are the two core results; net peptide content states how much of the fill weight is actually peptide.

A certificate belongs to a batch, not to a product line. When a new batch is released it is tested again, and the certificate published against it is that batch's certificate — a previous batch's result is never carried forward.

Is Metabolic Blend for human use?

No. This page describes structure and analysis. It makes no claim about what this compound does, and none should be inferred from it. Material is supplied for laboratory research use only: it is not a drug, food, dietary supplement, cosmetic or medical device, it is not for human or veterinary consumption, and no dosing, administration or reconstitution guidance is provided for any such use.

Common questions

What is in Metabolic Blend?

One vial contains NAD+ at 100 mg, MOTS-c at 10 mg and 5-Amino-1MQ at 10 mg. The ratio 100/10/10mg on the product states each named component's mass in that order, and the components are combined and dried together in that one vial rather than supplied as separate vials.

Does Metabolic Blend have a single purity figure?

No. Purity in the peptide sense describes one target measured against its own impurities, and this vial has 3 targets — two of which are not peptides, so the methods appropriate to them differ from the methods appropriate to the peptide. A coherent certificate reports an identity and a quantity for each named component, each with its own stated method.

Is Metabolic Blend supplied pre-mixed?

It is supplied as a single sealed vial of co-lyophilized powder: the named components are combined before drying, and nothing is supplied as a liquid or reconstituted before dispatch. Each component's mass is stated in the ratio on the product page and on its batch certificate.

How is each component of Metabolic Blend established?

Each named component is identified and quantified against its own reference standard, with the method stated beside the result. Read the certificate per analyte rather than as one number: one row per component, a stated method, and a result measured against a stated specification. A certificate that reports a single purity figure for a vial like this has characterised one component and implied it characterised all of them.

Is Metabolic Blend for human or veterinary use?

No. It is supplied for laboratory research use only, and it is not a drug, food, dietary supplement, cosmetic or medical device. No dosing, administration or reconstitution guidance is provided for any human or animal use, in any channel.

References

• MOTS-c — PubChem, National Library of Medicine