Compound reference
Wolverine Blend: structure and analysis
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Wolverine Blend is a two-compound research blend supplied for laboratory research. Each vial contains BPC-157 at 10 mg and TB-500 at 10 mg, combined and co-lyophilized into one vial, so it has no single molecular formula. This page covers its structure, how it is produced, and how identity and purity are established — for a batch, on that batch's certificate of analysis.
What is Wolverine Blend?
A two-compound blend supplied in a single vial: BPC-157 at 10 mg and TB-500 at 10 mg. The notation 10/10mg states each component's mass.
BPC-157 is a 15-residue synthetic peptide corresponding to a fragment of a protein found in gastric juice. TB-500 is the full 43-residue thymosin beta-4 sequence.
It is the simplest blend in the catalogue: two peptides, equal masses, no metal complex and no non-peptide components.
How is Wolverine Blend produced?
Supplied as a blend: the named components are combined and co-lyophilized into a single vial, with the mass of each component stated. The components are produced separately and characterised before combination.
This changes the analytical question. For a single compound, purity asks what share of the detected material was the target. For a blend there are several targets, and what matters is whether each named component is present at its stated mass — per-component identification and quantitation rather than one purity percentage.
What is in Wolverine Blend?
Wolverine Blend is supplied as one co-lyophilized vial holding 2 separately characterised components, in the ratio 10/10mg. A blend has no single molecular formula, because a formula describes one molecule and this vial holds several, so the table gives each component's own published figures.
For a neutral molecule the ion a mass spectrometer is compared against is the protonated species, [M+H]⁺. Two of these components are already charged — a copper complex and a quaternary ammonium — and for those the ion to compare against is the species itself; the table says which is which.
Two masses describe each of these molecules and they are different numbers: the monoisotopic mass, which assumes every carbon is carbon-12, and the tallest peak in the isotope envelope, which for a long peptide sits one or two carbon-13 substitutions above it. The table gives the monoisotopic figure; each component's own page gives both.
What a certificate can establish for a vial like this is a separate identity and a separate quantitation for each named component, each against a standard appropriate to that molecule. One purity percentage does not describe several targets, and the certificate has to be read per analyte rather than as one number.
How does Wolverine Blend compare with related compounds?
Related compounds are not interchangeable. Published figures differ between them, and in this catalogue three compounds share a GHRH backbone while several share the same structural class — which is exactly why identity is confirmed analytically rather than inferred from the label.
How is the identity of Wolverine Blend confirmed?
Two independent mass spectrometry confirmations. Fifteen residues against forty-three is a large enough difference that the two components are unambiguously distinguishable.
For TB-500 the identity result also has to confirm the full-length sequence rather than the short fragment sold under the same name, which is the substantive identity question in this vial.
Which impurities can appear in Wolverine Blend?
Two peptides differing threefold in length separate well chromatographically, so resolving the components from each other is straightforward. Resolving each from its own deletion products is the real work, and for a 43-residue peptide that is demanding.
Quantitation of each component at its stated 10 mg is the result that matters, rather than a single purity percentage.
Neither component contains methionine, cysteine or tryptophan, so the oxidation pathways that affect blends containing Semax or MOTS-c are not the primary concern here.
What does a certificate of analysis for Wolverine Blend report?
A certificate for a batch of this material names the batch, names the issuing laboratory, and states for each parameter both the method used and the specification the result was measured against. Identity and purity are the two core results; net peptide content states how much of the fill weight is actually peptide.
A certificate belongs to a batch, not to a product line. When a new batch is released it is tested again, and the certificate published against it is that batch's certificate — a previous batch's result is never carried forward.
Is Wolverine Blend for human use?
No. This page describes structure and analysis. It makes no claim about what this compound does, and none should be inferred from it. Material is supplied for laboratory research use only: it is not a drug, food, dietary supplement, cosmetic or medical device, it is not for human or veterinary consumption, and no dosing, administration or reconstitution guidance is provided for any such use.
Common questions
What is in Wolverine Blend?
One vial contains BPC-157 at 10 mg and TB-500 at 10 mg. The ratio 10/10mg on the product states each named component's mass in that order, and the components are combined and dried together in that one vial rather than supplied as separate vials.
Does Wolverine Blend have a single purity figure?
No. Purity in the peptide sense describes one target measured against its own impurities, and this vial has 2 targets. Each component's purity is meaningful only against its own reference standard, so a certificate for this blend reports per-component results rather than one percentage for the vial.
Is Wolverine Blend supplied pre-mixed?
It is supplied as a single sealed vial of co-lyophilized powder: the named components are combined before drying, and nothing is supplied as a liquid or reconstituted before dispatch. Each component's mass is stated in the ratio on the product page and on its batch certificate.
How is each component of Wolverine Blend established?
Each named component is identified and quantified against its own reference standard, with the method stated beside the result. Read the certificate per analyte rather than as one number: one row per component, a stated method, and a result measured against a stated specification. A certificate that reports a single purity figure for a vial like this has characterised one component and implied it characterised all of them.
Is Wolverine Blend for human or veterinary use?
No. It is supplied for laboratory research use only, and it is not a drug, food, dietary supplement, cosmetic or medical device. No dosing, administration or reconstitution guidance is provided for any human or animal use, in any channel.
References
- BPC-157 — PubChem, National Library of Medicine
- Thymosin beta-4 — PubChem, National Library of Medicine
- Thymosin beta-4 — human — UniProt Knowledgebase
From the catalog
Compounds referenced on this page
For laboratory research use only. Not for human or veterinary consumption.